Ongoing anthropogenic eutrophication of the catchment area threatens the Donana World Heritage Site (South-west Spain)

Eutrophication is a major cause of wetland degradation worldwide. In recent decades, reductions in nutrient inputs have led to improvements in water quality in many rivers and lakes in central and northern Europe, but long-term trends are less clear in southern Europe. We conducted the first comprehensive study of water quality in Donana (SW Spain), one of the most important wetland complexes in Europe and the Mediterranean region. The core area of Donana is a large shallow, seasonal marsh (UNESCO World Heritage Site-WHS) that floods during rainy, cool winter months, then dries out during the summer. The marsh is fed by three main streams whose catchments are within a Biosphere Reserve but are impacted by greenhouses (for cultivating fruit, irrigated with groundwater), poorly treated urban wastewaters and tourism. From 2013 to 2016, we monitored nutrient (Total P, Total N, PO4-3, NH4+, NO3-, NO2-) and phytoplankton chlorophyll-a (chla) concentrations in surface waters of the Donana marsh and the three main streams. We quantified changes in greenhouse cover since 1995 using satellite images. Nutrient concentrations in streams were consistently higher than in the marsh, particularly in the Partido and Rocina streams that regularly reached concentrations equivalent to a "bad physico-chemical status" under the EU Water Framework Directive (WFD), and whose catchments suffered a fivefold expansion of greenhouses from 1995 to 2016. The Partido was the most polluted stream, and the most affected by wastewater effluents, and had particularly high concentrations of NH4+ (Geometric Mean = 0.3 mg L-1) and NO2- (GM = 0.52 mg L-1) across seasons. Patterns in chla concentrations were less consistent, but streams (GM = 6.78 mu g L-1) generally had higher concentrations than the marsh (GM = 4.27 mu g L-1). Nutrient concentrations in spot samples within the marsh largely depended on a combination of evaporation (as revealed by higher stable isotope delta H-2 values in the water column) and spatial processes (concentrations increase close to stream entry points, where conductivity is lower). Anthropogenic nutrient pollution in entry streams is a serious problem in Donana, with extensive stretches too toxic for fish. Reinforcement of policies aimed at reducing nutrient inputs to Donana are urgently required to meet the biodiversity conservation and environmental objectives for the WHS and WFD, respectively. Paradoxically, the marsh is currently relied upon to purify the water entering from streams.