A Ketogenic Diet Extends Longevity and Healthspan in Adult Mice

Roberts, Megan N.; Wallace, Marita A.; Tomilov, Alexey A.; Zhou, Zeyu; Marcotte, George R.; Tran, Dianna; Perez, Gabriella; Gutierrez-Casado, Elena; Koike, Shinichiro; Knotts, Trina A.; Imai, Denise M.; Griffey, Stephen M.; Kim, Kyoungmi; Hagopian, Kevork; Haj, Fawaz G.; Baar, Keith; Cortopassi, Gino A.; Ramsey, Jon J.; Alberto Lopez-Dominguez, Jose

VL / 26 - BP / 539 - EP / +
Calorie restriction, without malnutrition, has been shown to increase lifespan and is associated with a shift away from glycolysis toward beta-oxidation. The objective of this study was to mimic this metabolic shift using low-carbohydrate diets and to determine the influence of these diets on longevity and healthspan in mice. C57BL/6 mice were assigned to a ketogenic, low-carbohydrate, or control diet at 12 months of age and were either allowed to live their natural lifespan or tested for physiological function after 1 or 14 months of dietary intervention. The ketogenic diet (KD) significantly increased median lifespan and survival compared to controls. In aged mice, only those consuming a KD displayed preservation of physiological function. The KD increased protein acetylation levels and regulated mTORC1 signaling in a tissue-dependent manner. This study demonstrates that a KD extends longevity and healthspan in mice.

Access level

Green accepted, Bronze