Strawberry (Fragaria x ananassa cv. Romina) methanolic extract attenuates Alzheimer's beta amyloid production and oxidative stress by SKN-1/NRF and DAF-16/FOXO mediated mechanisms in C. elegans
Navarro-Hortal, Maria D.; Romero-Marquez, Jose M.; Esteban-Munoz, Adelaida; Sanchez-Gonzalez, Cristina; Rivas-Garcia, Lorenzo; Llopis, Juan; Cianciosi, Danila; Giampieri, Francesca; Sumalla-Cano, Sandra; Battino, Maurizio; Quiles, Jose L.
Bioactive compounds from strawberries have been associated with multiple healthy benefits. The present study aimed to assess chemical characterization of a methanolic extract of the Romina strawberry variety in terms of antioxidant capacity, polyphenols profile and chemical elements content. Additionally, potential toxicity, the effect on amyloid-13 production and oxidative stress of the extract was in vivo evaluated in the experimental model Caenorhabditis elegans. Results revealed an important content in phenolic compounds (mainly ellagic acid and pelargonidin-3-glucoside) and minerals (K, Mg, P and Ca). The treatment with 100, 500 or 1000 mu g/mL of strawberry extract did not show toxicity. On the contrary, the extract was able to delay amyloid 13-protein induced paralysis, reduced amyloid-13 aggregation and prevented oxidative stress. The potential molecular mechanisms present behind the observed results explored by RNAi technology revealed that DAF-16/FOXO and SKN-1/NRF2 signaling pathways were, at least partially, involved.
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